ORDER & PROTOCOLS
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2020 version
2019 version
**
Requierment for
synthesis
of PLGA
NPs (Datasheet with PDF file)
Contact us: info.nanoglia@gmail.com, visnu528@gmail.com
1.Send the materials which will be loaded into the PLGA NPs.
1.Send the materials which will be loaded into the PLGA NPs.
Recommended
at
least amount of the materials
siRNA,
miRNA:
200
μl
solution of 20 μM
plasmid
DNA: 100 μg
CRISPR/Cas9:
10
- 20 mg (In case of Cas9 mRNA)
2.The size
and zeta potential, verifying successful synthesis of PLGA NPs are provided
without compensation.
3.To
confirm the localization after injection in vivo, AAV-mCherry loaded
PLGA NPs are provided without compensation.
Experimental Protocols
You can receive
10 vials each containing 2.5
mg PLGA nanoparticles
* The
concentration
of nanoparticles for optimal using
varies by application. The initial conditions suggested here are guidelines
that may be modified based on the tissue or animal models.
1.
Preparing solutions
- Completely dissolve PLGA nanoparticles in
250 μl of
autoclaved PBS and mix immediately by
vortex mixer. (Do not centrifuge)
- If PLGA
nanoparticles are perfectly not dissolved, sonicate the sample in 30w for 15 s.
This may induce the break of PLGA nanoparticles.
2.
General Guidelines : Use working concentrations of
0-200
μg/ml.
3.
Before use the sample, wait for at least 30s at room temperature (15-25℃) to
sink unmelted PLGA
nanoparticles
4.
Use the upper lysate to applicate in vivo.
